Active substances: Norfloxacin
Key words: Psychrophilic bacteria, Arthrobacter psychrolactophilus, cold-active lactose hydrolyzing enzyme, Mt.
Recently a wide diversity of cold adapted microorganisms have been found in various cold environments Groudieva et al. Owing to the fact that cold completely penetrates microorganisms, all components including enzymes of psychrophiles living in cold environment must be suitably adapted.
E-mail: ajk knou. A variety of useful cold active enzymes have been found in psychrophiles Feller et al.
Cold active enzymes are attractive in food industry. Cold active enzyme hydrolyzing lactose to glucose and galactose at refrigerating temperature is an important food-industrial enzyme, because it can be used to produce lactose hydrolyzed milk for the lactose intolerant people, and can also be used to convert lactose in whey, a by-product of cheese industry, to more readily fermentable glucose and galactose.
Until now, some psychrophilic bacteria producing -galactosidase have been reported, but none of them have been hitherto used practically in the food industry, meaning that more study is required to find better microbial source.
The aim of this study was to isolate a psychrophilic bacterium, from Mt. Himalaya of Nepal, producing the cold-active lactose hydrolyzing enzyme practically useful in food industry.
Himalaya of Nepal by using sterilized awls, sterilized spoons and sterilized Falcon tubes. Morphological, biochemical and physiological properties Isolates showing good growth and good X-gal hydrolysis on BHI agar, and high activity for hydrolysis of ONPG and lactose were investigated for Gram staining, morphological, biochemical, and physiological properties.
The fatty acids were analyzed by gas chromatography Hewlett Packard 6890, USA and identified using the microbial identification software package Sasser. Indira. Jayalakshmi.